Effect of arsenic on cell growth of the cellular slime mould, Dictyostelium discoideum.
نویسندگان
چکیده
The growing D. discoideum cells were killed in a dose-dependent manner when exposed to 100 and 140 ppm of arsenic (As2O3) at mid-log phase for 20 min. Reduced plaque sizes and changed cell and colony morphologies were observed in the treated cells. Endocytotic functions (both phagocytosis and pinocytosis) were also inhibited in the treated cells. Arsenic treated cell showed a lower DNA and protein synthetic activities. These findings are discussed in relation to known mechanism of action of the heavy metal on growth-related cellular functions.
منابع مشابه
Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture.
1. A simple axenic medium suitable for the growth of the myxamoebae of a strain of the cellular slime mould Dictyostelium discoideum is described. 2. Procedures suitable for the growth of this strain in liquid and on solid media are described. 3. Conditions suitable for initiating the cell differentiation of myxamoebae grown axenically are described.
متن کاملControl of cell differentiation in the cellular slime mould Dictyostelium discoideum.
s of Communications 565th Meeting of the Biochemical Society University of Stirling 9 and 10 September 1976 CONTROL M ECH AN ISMS I N D I FFER ENTl AT1 ON AND DEVELOPMENT: a Colloquium organized by L. Stevens (Stirling) Control of Cell Differentiation in the Cellular Slime Mould Dictyostelium discoideum
متن کاملCell electrophoresis of the cellular slime mould Dictyostelium discoideum. I. Characterization of some of the cell surface ionogenic groups.
The reduction in cell surface charge density during the early stages of differentiation of the cellular slime mould, Dictyostelium discoideum, has been studied by the technique of cell electrophoresis. Using specific chemical reagents, it was shown that the cell surface negative charge was probably due to side chain carboxyl groups of proteins. During differentiation, there was a decrease in th...
متن کاملCell electrophoresis of the cellular slime mould Dictyostelium discoideum. II. Relevance of the changes in cell surface charge density to cell aggregation and morphogenesis.
The reduction in the cell surface charge density of Dictyostelium discoideum during differentiation has been studied by the technique of cell electrophoresis. It was abolished under conditions in which cell aggregation was inhibited (e.g. low temperature, the presence of actinomycin D or cycloheximide). In the presence of nutrients incapable of supporting growth, cell aggregation occurred witho...
متن کاملA neutral ceramidase homologue from Dictyostelium discoideum exhibits an acidic pH optimum.
The nucleotide sequence reported for the Dictyostelium discoideum ceramidase is available on the DNA Data Bank of Japan (DDBJ). Ceramidases (CDases) are currently classified into three categories (acid, neutral and alkaline) based on their optimal pHs and primary structures. Here, we report the first exception to this rule. We cloned the CDase cDNA, consisting of 2142 nucleotides encoding 714 a...
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عنوان ژورنال:
- Indian journal of experimental biology
دوره 41 11 شماره
صفحات -
تاریخ انتشار 2003